Radioimmunoassay of Human Urinary Kallikrein.
نویسندگان
چکیده
منابع مشابه
Enzyme Immunoassay of Human Urinary Kallikrein
An enzyme immunoassay for the determination of human urinary kallikrein has been developed and is compared with other human urinary kallikrein assays such äs radioimmunoassay, dog blood pressure assay, rat uterus test after kinin liberation and synthetic Substrate) tests (AcPheArgOEt and S-2266). The usable ränge of the Standard curve is from 0.05 to 12 ng kallikrein per test. The intfaassay co...
متن کاملStructural studies of human urinary kallikrein (urokallikrein).
Human urinary kallikrein (urokallikrein) has been purified by affinity chromatography with aprotinin coupled to CH-Sepharose and by gel filtration. The isolation procedure, which was performed under mild conditions, was completed in a 36-hr period and yielded an overall recovery of more than 75% and a purification of 1727-fold. Homogeneity of the urokallikrein was demonstrated by three criteria...
متن کاملHuman urinary kallikrein - biochemical and physiological aspects.
The h i s t o r y of k a l l i k r e i n begins w i t h the d i s c o v e r y of u r i n a r y k a l l i k r e i n more than f i f t y years ago. The r e n a l o r i g i n of u r i n a r y k a l l i k r e i n , though not f i n a l l y proved, i s l a r g e l y accepted. A p o s s i b l e r o l e of k a l l i k r e i n i n the r e g u l a t i o n of kidney f u n c t i o n and blood pressure has...
متن کاملSpecificity of substrate analogue inhibitors of human urinary kallikrein.
A series of acetyl-peptidyl-amides containing the amino acid sequence around the Arg-Ser kallikrein cleavage site of bovine kininogen were synthesized and tested for their ability to inhibit both the kinin-releasing activity and the amidase activity of purified human urinary kallikrein. The substrate analogues were competitive inhibitors for human urinary kallikrein and the heptapeptides (P4-P3...
متن کاملLoss-of-function polymorphism of the human kallikrein gene with reduced urinary kallikrein activity.
Kallikrein is synthesized in the distal tubules and produces kinins, which are involved in the regulation of vascular tone in the kidney. Urinary kallikrein activity has been reported to be partly inherited and to be reduced in essential hypertension. In a systematic search for molecular variants of the human kallikrein gene, nine single-nucleotide polymorphisms were identified. Five of those p...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1980
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1980.18.7.395